5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
Fig 2
PpIX accumulation in GIST-T1 cells in vitro.
GIST-T1 cells were loaded with PpIX for different durations. Accumulation of PpIX within cells was analyzed using FACS with excitation at 405 nm and emission at 680 nm. (A) Histogram data from FACS. The abscissa (X-axis) indicates intensity of emission and the ordinate (Y-axis) represents number of cells. The green and red histograms represent cell samples at 0 min (as a control) and cell samples at each indicated time point, respectively. (B) Temporal changes in mean fluorescence intensity (M.F.I.). The abscissa (X-axis) indicates time and the ordinate (Y-axis) of the graph represents M.F.I.