Matrix Metalloproteinase-9 (MMP-9) induced disruption of intestinal epithelial tight junction barrier is mediated by NF-κB activation
Fig 4
Effect of MMP-9 on Caco-2 cell death.
(A) Dual-color dot plots representing cellular apoptosis. Upper left panel, Necrotic cells; lower right panel, apoptotic cells. Caco-2 monolayers were treated with MMP-9 for 24 and 48 hrs. Caco-2 monolayers were labeled with Annexin VFITC (apoptosis) or 7-AAD (necrosis). MMP-9 did not induce apoptosis or cell necrosis in Caco-2 monolayers. (B) % of live cell detection by trypan blue exclusion method. MMP-9 treatment did not affect total number of dead Caco-2 cells compared to control.