A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids
Fig 2
Mito-bulbs in A549 cells exhibit poor inner membrane organization in matrix regions containing clustered mtDNA nucleoids.
A. SIM image depicts mito-bulbs in a DMEM-adapted A549 cell expressing MLS-EGFP (blue) enclosed by markers for the outer membrane (Tom20, red) and inner membrane (PHB2, green). The yellow boxed ROI is expanded and channels resolved in panels on the right. Scale bar is 2.5 μm. B. SIM image of a DMEM-adapted A549 cell stained with antibodies against outer membrane Tom20 (red), inner membrane marker ATPB (blue) and DNA (green), shown as individual channels on the right. Scale bar is 5 μm.