Organic synthesis and anti-influenza A virus activity of cyclobakuchiols A, B, C, and D
Fig 5
Cyclobakuchiols A–D inhibits influenza A viral growth.
MDCK cells were infected with A/PR/8/34 (A) or A/WSN/33 (B) viruses prior to the addition of cyclobakuchiol A (2), B (3), C (4), and D (5) (12.5 μM; n = 9), DMSO (0.125%; n = 9) or (+)-(S)-bakuchiol (1) (12.5 μM; n = 9). The conditioned culture media were collected at the indicated time-points and added to MDCK cells, and the treated cells were immunostained. The viral titers were calculated from the number of stained cells. Data represent the mean ± SEM and are representative of three independent experiments. **p < 0.01, ***p < 0.001 for the comparison of DMSO-treatment. Results were reproducible in this experiment.