Organic synthesis and anti-influenza A virus activity of cyclobakuchiols A, B, C, and D
Fig 2
Toxicity of cyclobakuchiols A–D against MDCK cells.
The indicated concentrations of cyclobakuchiols A (2), B (3), C (4), and D (5) or (+)-(S)-bakuchiol (1) in DMSO (concentrations of 100 μM, 1%; 50 μM, 0.5%; 25 μM, 0.25%; 12.5 μM, 0.125%; 6.3 μM, 0.063%; 3.1 μM, 0.031%; 1.6 μM, 0.016%; 0.8 μM, 0.008%) were added to the MDCK cells. Cell viabilities were determined by MTT assay after incubation for 24 h (n = 5 each) (A) or 72 h (n = 5 each) (B). Data represent the mean ± SEM and were representative of three independent experiments. ***p < 0.001, for the comparison with DMSO-treatment. The results were reproducible in this experiment.