Selective killing of homologous recombination-deficient cancer cell lines by inhibitors of the RPA:RAD52 protein-protein interaction
Fig 2
BRCA1-deficient HCC1937 breast cancer cell line survival assay.
BRCA1-deficient HCC1937 cells corrected with wild-type BRCA1 gene (BRCA1+; closed triangle), and with an empty vector (BRCA1-; closed circle) were examined. For each experiment (Figs 2–4), cell lines were treated with nine concentrations of (A) quinacrine, (B) mitoxantrone, and (C) doxorubicin in 96-well culture plates at 5x103 cell/well density. Each treatment point was made in sextuplicate. Treated cells were incubated for 72 hours at 37°C before assessing cell viability using PrestoBlue. Data were normalized to vehicle control. The experiment was repeated three times. Error bars indicate the standard deviation. Significantly different data are indicated with an asterisk. EC50 values, estimated from the graphs, are given.