Phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth
Fig 4
Acquisition of single-layer agar assay through lensless imaging.
(a) Image of a single-layer soft agar assay after 21 hr 44 minutes of incubation at 37°C, with the dark background corresponding to the area of bacterial growth and bright disks to lysis plaques. The orange arrows indicate plaques that were not excluded from the growth kinetics study. (b) Corresponding plaque radius histograms and (c) cumulative graph of plaques detected as a function of time.