Gm14230 controls Tbc1d24 cytoophidia and neuronal cellular juvenescence
Fig 5
Tbc1d24 cytoophidia formation is enhanced in zeocin-induced loss of cellular juvenescence.
(A) The appearance of Neuro2a cells 72 hrs after treatment with zeocin. Scale bar = 100 μm. (B) Number of cells per field 72 hrs after treatment with or without zeocin. (C) Immunofluorescence analysis of Tbc1d24 and Vimentin treated with or without zeocin for 72 hrs. Scale bar = 25 μm. (D) Frequency of cytoophidia in Neuro2a cells with or without zeocin treatment for 72 hrs. (E) qPCR analysis of Tbc1d24 in Neuro2a cells transfected with control siRNA or Tbc1d24 siRNA. Data were normalized to Tubb5 (n = 3). (F) The appearance of Neuro2a cells treated with or without zeocin for 96 hrs and simultaneously transfected with control siRNA or Tbc1d24 siRNA. Scale bar = 100 μm. (G) Number of cells per field 96 hrs after treatment with or without zeocin. (H) The appearance of cells with Sytox blue staining in the same fields as in (F). The gray color dots indicate dead cells stained with Sytox blue. (I) Frequency of Sytox blue-positive cells. **p < 0.01; Student’s t-test. The data were presented as the means ± SEM.