Gm14230 controls Tbc1d24 cytoophidia and neuronal cellular juvenescence
Fig 2
Tbc1d24 cytoophidia are distinct from Impdh and Ctps cytoophidia.
(A) Costaining of Impdh and Tbc1d24 in Neuro2a cells. Scale bar = 25 μm. (B) Costaining of Ctps and Tbc1d24 in Neuro2a cells. Scale bar = 25 μm. (C) Immunofluorescence analysis of Tbc1d24 in Neuro2a cells treated with control distilled water or 2 mM 6-diazo-5-oxo-L-norleucine (DON) for 24 hours (hrs). Scale bar = 25 μm. (D) Frequency of Tbc1d24 cytoophidium-positive Neuro2a cells treated with 2 mM DON for 24 hrs. (E) Immunofluorescence analysis of Tbc1d24 in Neuro2a cells treated with control dimethyl sulfoxide (DMSO) or 2 μM mycophenolate (MPA) for 24 hrs. Scale bar = 25 μm. (F) Frequency of Tbc1d24 cytoophidium-positive Neuro2a cells treated with 2 μM MPA for 24 hrs. (G) Immunofluorescence analysis of Tbc1d24 in Neuro2a cells treated with control distilled water or 2 mM Acivicin for 24 hrs. Scale bar = 25 μm. (H) Frequency of Tbc1d24 cytoophidium-positive Neuro2a cells treated with 2 mM Acivicin for 24 hrs. **p<0.01; Student’s t-test. The data were presented as the means ± SEM.