Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
Fig 2
(A) Four example serial sections (each cut at 7 μm thick) from the same 2 mm tissue block, and changes to both bright light (top) and polarized light (bottom) images are observed in the cap region. (B) Four different sections (numbered from left to right) from 3 different hearts showed increased repeatability in (C) with pixel counting method compared to use of grayscale intensity. Scale bars = 1 mm.