Small molecule allosteric inhibitors of RORγt block Th17-dependent inflammation and associated gene expression in vivo
Fig 5
IMQ-induced skin inflammation and RORγt activity is inhibited by Compound 3.
Ear thickness (mm) was measured in naïve or IMQ-treated animals on day 4 using digital micro-calipers (A). Th17 cytokine gene expression analysis was performed for Il17a (B), Il17f (C) and Bclxl (D) on day 4. Expression is normalized to Gapdh and presented as fold change over naïve. Kinetic assessment of plasma concentration of Compound 3 (E). Each symbol represents an individual animal and error bars denote mean ± SEM. Statistical significance (*p ≤ 0.05) was determined using one-way ANOVA with Tukey’s multiple comparisons test, *significant over naïve; **significance over vehicle-treated group. Data are representative of 1–4 independent experiments with n = 6-24/group.