Anoctamin 2-chloride channels reduce simple spike activity and mediate inhibition at elevated calcium concentration in cerebellar Purkinje cells
Fig 6
ANO2 increases the hyperpolarization following strong activation of Purkinje cells.
(A) Representative traces of the afterhyperpolarization (AHP) following strong activation of wildtype (black/grey) and Ano2-/- (red/dark red) Purkinje cells at physiological and elevated ECl. The holding current was set at -500 pA or -550 pA for all cells in this example. (B) AHP amplitude after strong activation is significantly larger in wildtype compared to and Ano2-/- mice at physiological ECl, but not at elevated ECl (ECl -87 mV: wt -11.2 ± 2.8 mV, n = 20 cells of N = 6 animals; Ano2-/- -8.9 ± 2.4 mV, n = 19 N = 7; Student’s t-test p = 0.0097; ECl -48 mV: wt -9.0 ± 3.0 mV, n = 27 cells of N = 6 animals; Ano2-/- -9.2 ± 1.9 mV, n = 31 N = 7; Student’s t-test p = 0.7983) (C) Time till the maximal AHP is reached after strong activation is unaltered by ANO2 or the elevation of ECl (ECl -87 mV: wt 187.8 ± 53.2 ms, n = 20 cells of N = 6 animals; Ano2-/- 178.1 ± 35.6 ms, n = 19 N = 7; Student’s t-test p = 0.5073; ECl -48 mV: wt 172.4 ± 50.7 mV, n = 27 cells of N = 6 animals; Ano2-/- 181.4 ± 49.8 ms, n = 31 N = 7; Student’s t-test p = 0.5035). ** p < 0.01.