Anoctamin 2-chloride channels reduce simple spike activity and mediate inhibition at elevated calcium concentration in cerebellar Purkinje cells
Fig 5
ANO2-mediated chloride currents gradually increase interspike intervals.
Progression of interspike intervals (ISIs) during the first sixteen simple spikes upon moderate (A) and strong activation (B) of Purkinje cells in wildtype (black) and Ano2-/- mice (red). At physiological ECl (left), the interspike intervals in wildtype mice grow longer from the second ISI on, causing a divergence of the ISIs from wildtype and Ano2-/- mice. In contrast, the progression of ISIs at elevated ECl (right) develops without divergence (A left: Genotype effect: F(1, 930) = 22.65, p < 0.001; ISI number x genotype interaction: F(14, 930) = 0.09, p = 1; A right: Genotype effect: F(1, 900) = 0.18, p = 0.6712; ISI number x genotype interaction: F(14, 930) = 0.01, p = 1; B left: Genotype effect: F(1, 570) = 29.37, p < 0.001; ISI number x genotype interaction: F(14, 570) = 0.14, p = 1; B right: Genotype effect: F(1, 840) = 4.2, p = 0.0430; ISI number x genotype interaction: F(14, 840) = 3.7, p = 1). * p < 0.05; *** p < 0.001.