Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
Fig 3
Her2-bridging CAR-CD19 T cells are cytotoxic against both CD19-positive and Her2-positive cells in vitro.
(A, B) Example of the flow cytometry gating strategy (A) and the ELISA assay used (B) to determine the purity and phenotype of transduced human donor CAR-CD19 T cells that secrete a bridging protein. C) Her2-bridging CAR-CD19 T cells were added at different E:T ratios to Nalm6 cells (left) or SKOV3 cells (right) and cytotoxicity was measured. E:T ratios used were 10:1 (red bars), 3:1 (light blue bars) and 1:1 (black bars). The donor matched UTD cells are also shown at each E:T ratio.