Kola nut from Cola nitida vent. Schott administered to pregnant rats induces histological alterations in pups’ cerebellum
Fig 5
Mid-sagittal sections of formalin-fixed, paraffin-embedded, H&E stained cerebellum of PND 14 pups.
CON shows a normal molecular layer (ML) with few cells scattered in the parenchyma. TRT1 shows thinner ML with a mild separation between the ML and granular layer (black arrow) with pyknotic and degenerated purkinje cells (black arrow heads). TRT2 shows thinner ML with sparse Purkinje cells (black arrow). TRT3 shows relatively normal ML with various morphologies (red thin arrow) in the parenchyma, slight separation of the ML and granular layer (black arrow), and pyknotic purkinje cells with central chromatolysis (black arrow head) Scale bar = 40 μm.