Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrix
Fig 6
Metabolic measurements in MSCs.
A) Mitochondrial respiration profile (OCR) of AT-and UC-MSCs under basal conditions and after the addition of 1.5 μM oligomycin, 2.0 μM and 0.5 μM FCCP and a mixture of rotenone plus antimycin A (0.5 μM) injections where indicated by the dotted lines. B) Mitochondrial parameters (basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity) in AT- and UC-MSCs. C) Basal ECAR and ECAR changes after addition of the mitochondrial inhibitors: 1.5 μM oligomycin, 2.0 μM and 0.5 μM FCCP and a mixture of rotenone plus antimycin A (0.5 μM) addition on AT- and UC-MSCs. Data expressed as points (A, C) and column chart (B) represent the mean ± SD (vertical bars) from three experiments carried out on different cell preparations. * indicates significant differences (P<0.05).