Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrix
Fig 5
Real-Time ATP Production Rate Assay of basal OCR (blue spheres) and ECAR (blue squares) rates of AT-MSCs (A) and OCR (orange spheres) and ECAR (orange squares) rates of UC-MSCs (B). After detection of OCR and ECAR rates, oligomycin (1.5 μM) and rotenone plus antimycin A (0.5 μM) were serially injected at fixed times in order to allow to detect the mitochondrial and glycolytic ATP production rates. C) AT- and UC-MSCs quantification of ATP production by mitochondrial oxidative phosphorylation (dashed rectangle) or by the glycolytic pathway (glucose conversion to lactate) (filled rectangle). D) Energy map of AT-MSCs and UC-MSCs with OCR vs ECAR of AT- (blue spheres) and UC-MSCs (orange spheres) are plotted. E) The plot shows the ratio between the mitochondrial ATP production rate and the glycolytic ATP production rate (logarithmic scale). Data expressed as points (A, B, D, E plots) and column chart (C plot) represent the mean ± SD (vertical bars) from three experiments carried out on different cell preparations. * indicates significant differences (P<0.05).