Comparison of gamma and x-ray irradiation for myeloablation and establishment of normal and autoimmune syngeneic bone marrow chimeras
Fig 5
Chimerism of 564Igi mixed chimeras.
A) Schematic overview of flow cytometry gating tree, gating layout can be seen in S2B Fig. Percentages of chimerism in blood (B), IngLN (C), MesLN (D) and spleen (E) after irradiation at doses of 9 and 11 Gy using either X-ray or Cs-137 irradiation. CD45.1 positive cells derive from the donor mice and CD45.2 cells are residual recipient cells or derive from the 564Igi donor compartment. Naïve mice have not been exposed to any treatment. In all bar graphs, the bar indicates the mean and error bars indicate the standard error of mean. Based on two-way ANOVA, irradiation modality only came out as an independent source of variation in the data for the NK cell population in MesLN (P = 0.0321), but in all cases there was a significant interaction effect (P<0.0001 throughout).