Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field
Fig 3
Degree of quenching against concentration.
All of the curves were fitted with the modified Stern-Volmer equation for self-quenching: . The value of ν was 2.6, for Sulforhodamine B, 3.0 for FTIC and 0.04 for calcein.