Cholangiocyte Epithelial to Mesenchymal Transition (EMT) is a potential molecular mechanism driving ischemic cholangiopathy in liver transplantation
Fig 5
Mesenchymal protein expression profiles on cholangiocytes.
Cholangiocyte expression (via western blot) of Vimentin (panel A), SNAIL (panel B), and N-Cadherin (panel-C) in Naive cholangiocytes, from those cold stored for 24 hours (CS), and from those with both 60 minutes of warm ischemia and 24 hours of cold storage (WI+CS). Expression is shown for 24 hours, 1, 2, and 3 weeks from ischemia exposure to staining. Panel D shows an actual chemiluminescent western blot analysis of these proteins and the GAPDH loading control. All samples were loaded at 30 μg protein per lane. *P<0.05, values are expressed as mean +/- SD from 5 independent experiments.