Comparative phenotypic, genotypic and genomic analyses of Bacillus thuringiensis associated with foodborne outbreaks in France
Fig 2
Nhe and Hbl production by a representative panel of Bt isolates composed of FBO-Bt isolates from group A (n = 21) and commercial Bt strains (n = 10).
(A) Nhe production was expressed as Nhe absorbance (414nm), corresponding to the coloration intensity obtained with the BDE VIATM kit (3M-Tecra), according to the manufacturer’s recommendations. (B) Hbl dilution values correspond to the highest dilution for which Hbl remained detectable, using the immunoenzymatic kit BCET-RPLA (Oxoid), and according to the manufacturer’s recommendations. Dilution = 1 is the limit of detection of Hbl and corresponds to 2 ng/mL of Hbl components. The value indices obtained for FBO-Bt and commercial Bt were compared using the non-parametric Wilcoxon statistical test. ns = not significant.