Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
Fig 5
The gene expression and phenotype of overexpression TaAAP13D under the control of endosperm–specific promoter HMW-GS 1Dx5.
(a), Gene expression of TaAAP13 at 21DPA in developing grains of overexpression (OE) lines: P22-OE and P16-OE (6 copies), P25-OE (16 copies), P26-OE (14copies), P23-OE (26 copies), P24 (12 copies), null (P15-null), and non-transgenic (P29-WT, Cadenza). Significant differences between OE lines and null or wild type were detected (F2,14 = 20.85, p<0.001) (P<0.001, F-test) and indicated with asterisk (***). (b), N concentration % of wholemeal flour. (c), Thousand grain weight (TGW) at 15% moisture content (g). (d), Nitrogen content (mg) per grain. (e), Grain morphology of T3 grains. Scale bar represents 1 cm. Significant differences between OE lines and null were detected using ANOVA (P<0.05, F-test) and indicated with asterisk (*).