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Bisphenol-C is the strongest bifunctional ERα-agonist and ERβ-antagonist due to magnified halogen bonding

Fig 2

Competitive radio-ligand receptor-binding assays.

Dose-response receptor binding curves are shown for the assays for (A) ERα and (B) ERβ. 17β-estradiol (E2) was used as an internal standard compound in the assays for both ERα and ERβ, in which tritiated [3H]E2 was used as a radiolabeled receptor tracer. The Y axis is expressed by the normalized binding data from 100% (no competitor chemical) to 0% (nonspecific binding at maximal concentrations of competitor). Log[chemicals (M)] is the logarithm of the concentration of competitor chemicals plotted on the X-axis.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0246583.g002