Preclinical testing of small diameter Descemet membrane endothelial keratoplasty grafts to increase tissue availability
Fig 2
In vitro surgery performed with the small diameter DMEK graft.
(A) Graft staining with 0.06% trypan blue. (B) Graft loading into a straight DMEK injector. (C) Descemetorhexis performed on a cornea mounted on an artificial anterior chamber. (D) Graft unfolding and positioning in the descemetorhexis area performed in a whole globe. Purple dashed line indicates the outline of the descemetorhexis, and the blue dashed line indicates the position of the 4mm DMEK graft. (E) AS-OCT graft imaging after 30–45 minutes of a fully pressurized anterior chamber.