Short- and long-term memory of moving amoeboid cells
Fig 7
Kinetics of memory of polarity axis.
Wild-type AX3 cells expressing Raf-RBD-GFP are moving in buffer. At t = 0 seconds the cells extend a de novo pseudopod, generating a NEW front. Pseudopod activity (seize and frequency) was measured in the New and Old front, as well as the time of retraction (T) of the pseudopod in the Old or the New front. Panels a show an example in which both the Old and New front exhibit pseudopod activity. Panels b show an example in which only the New front exhibits pseudopod activity. c. All 98 cases of a de novo pseudopod were analyzed for the retraction of either the Old or the New front. d. Detailed analysis of the subset of 28 cases in which only the New front exhibits pseudopod activity and the pseudopod in the Old front is retracted at time T. The experimental data were fitted to Eq (7) yielding a half-time of learning (25.7 ± 4.6 s) and forgetting (88 ± 43) of the polarity axis (optimal fit and 95% CI). The full line is the optimal fit, the dashed lines give the 95% confidence levels.