Short- and long-term memory of moving amoeboid cells
Fig 6
Kinetics of signaling molecules in pseudopods of a new or a retracting polarity axis.
Wild type AX3 cells expressing different markers were analyzed. a. Cells begin to extend a de novo pseudopod at the side of the cell forming a new polarity axis. Time t = 0 is the start of the pseudopod. Indicated are the times at which the sensors for the indicated signaling molecules begin to increase or decrease at the place where the pseudopod later begins. Data are means and SEM of 8 determinations. b. Cells with two extending pseudopods retract one pseudopod. The extension of one pseudopod stops at time t = x and retraction begins at t = 0s. The value of x, is very variable, between 4 and 20 s, but in all cases F-actin declines about 3 s before the pseudopod stops. Data are means and SEM of 10 determinations. c. Images of a cell expressing LimE-GFP (detecting F-actin) and myosin-RFP that retracts the upper pseudopod. The yellow dot is at identical positions in the three images. The upper pseudopod begins retraction at 50s.