Single stranded DNA annealing is a conserved activity of telomere resolvases
Fig 5
TelA can anneal plasmid length ssDNA.
0.7% agarose/ 1X TAE gel panels showing plasmid annealing reactions. The positions of heat denatured pUC19 (ssDNA), unit-length plasmid duplex (dsDNA) and the wells are labeled to the right of the gel. Reaction timepoints and the presence or absence of reaction components are indicated in the key above the gel. 76 nM of TelA was incubated with 1.78 mM nucleotides of the plasmid substrate in buffer containing 25 mM HEPES (pH 7.6), 2 mM MgCl2, 1 mM DTT, 100 μg/mL BSA and 50 mM potassium glutamate. pUC19 was linearized, 32P-endlabeled and denatured as described in the Materials and Methods section.