Design of a diagnostic system based on molecular markers derived from the ascomycetes pan-genome analysis: The case of Fusarium dieback disease
Fig 5
PCR analysis of the markers designed for Fusarium kuroshium diagnosis.
From left to right, every three lanes correspond to each of the different markers (FuKu01, FuKu02, and FuKu03, respectively). These markers were tested using genomic DNA from different species of the Fusarium genus (A) and another phytopathogenic ascomycete fungi (B). Tested species were: On (A), F. kuroshium ([Fk]; lanes 1–3), F. graminearum ([Fg]; lanes 4–6), F. verticillioides ([Fv]; lanes 7–9), F. oxysporum ([Fo]; lanes 10–12), F. tricinctum ([Ft]; lanes 13–15), and F. solani ([Fs]; lanes 16–18); while on (B), Fusarium kuroshium ([Fk]; lanes 1–3), A. alternata ([Aa]; lanes 4–6), B. cinerea ([Bc]; lanes 7–9), and N. parvum ([Np]; lanes 10–12).