The C-terminal domain of the type III secretion chaperone HpaB contributes to dissociation of chaperone-effector complex in Xanthomonas campestris pv. campestris
Fig 5
The C-terminal domain of HpaB is dispensable for self-interaction in Xcc.
(A-B) GST and GST-fused HpaB derivates were immobilized on MagneGSTTM particles and incubated with cell lysate containing Trx-6×His-HpaB, respectively. Samples were analyzed by immunoblotting with hexahistidine-specific antibody and GST-specific antibody. (C) GST, GST-HpaB, and GST-HpaB1-136 were immobilized on MagneGSTTM particles and incubated with cell lysate containing Trx-6×His, as negative control. (D) GST and GST-fused HpaB derivates were respectively immobilized on MagneGSTTM particles and incubated with cell lysate containing 6×His-HpaB1-144.