Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages
Fig 5
Model depicting the inhibitory effect of AGE-3 on the response to LPS in macrophages.
LPS binding to CD14 is endocytosed via the formation of a dimerised TLR4/MD-2 complex. Subsequently, this assembly activates TRIF signalling on endosomes and the transcription of IFN-inducible genes including CXCL10. AGE-3 induces the downregulation of CD14 expression though RAGE. Downregulation of CD14 expression induced by AGE-3 contributes to the decreased LPS uptake and CXCL10 release in macrophages.