The high expression of NUDT5 indicates poor prognosis of breast cancer by modulating AKT / Cyclin D signaling
Fig 5
The inhibition of AKT phosphorylation and Cyclin D1 expression in breast cancer cells by Knocking down NUDT5.
A, Representative images of MCF-7 and T47D cells with shNUDT5/empty vector (shScramble) and MDA-MB-231 cells with siNUDT5/siControl NUDT5. The expression of AKT, p-AKT, ERK, p-ERK, and cyclin D1 was analyzed by western blotting. B–D, Quantified expression of NUDT5, AKT, p-AKT, ERK, p-ERK, and cyclin D1 normalized to β-actin and scramble/negative control in MCF-7, T47D, and MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.