Comparison of extracellular vesicle isolation and storage methods using high-sensitivity flow cytometry
Fig 5
Effect of storage at 4°C or -80°C on EV concentrations.
EV number concentrations were evaluated using (A) scatter-based NTA and (B) fluorescence-triggered HS-FCM on fresh isolates obtained using DC + UC or exoEasy, and after 1 week, 2 weeks and 1 month of storage. A representative experiment out of two is shown. The NTA concentrations were determined in triplicate and results are shown as the average ± standard deviation. For the HS-FCM measurements, the total number of particles in 30 seconds was determined.