Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell
Fig 5
3-AT suppresses inflammatory response through promoting the formation of 4HNE-IκBα adduct.
(A) Cells pre-treated with 3-AT for 12 h were further incubated with or without LPS for additional 12 h. Cells were then subjected to immunoblotting with anti-IκBα and anti-phospho-IκBα. (B) Cells were treated as in (A), followed by subcellular fractionation, and immunoblotting for NF-κB, β-actin and CREB. Cells were treated as in (A) and mRNA expression levels pro-inflammatory genes were measured by qPCR. All error bars represent the mean ± S.D. (n = 3, independent experiments), *p < 0.05.