Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell
Fig 2
Scavenging of peroxisomal ROS prevented the 3-AT mediated degradation of peroxisome matrix proteins.
(A) Cells treated with H2O2, 3AT (10 mM), NAC (2 mM) or co-treatment with NAC and 3-AT for 24 h. Cells were then treated with DCFH-DA (1 μM) and examined under fluorescence microscope. (B) Bar graph showing the fluorescent intensity of DCFH-DA from (A). (C) Cells were transiently transfected with HyPer-SKL plasmid for 24 h and then treated with 3AT, for 24 h. (D) Peroxisomal ROS production was measured by fluorescence intensity of HyPer-SKL in the transfected cells from (C). (E) Cells were treated with 3-AT alone, combination of 3-AT and NAC or NAC alone and followed by immunoblotting. (F) Densitometric analysis of band from (E). (G) Cells were treated as in (E) and immunostaining was performed with anti-catalase and anti-PMP70. Representative images are shown. All error bars represent the mean ± S.D. (n = 3, independent experiments), *p < 0.05, ns: non significance.