Mycobacterium smegmatis does not display functional redundancy in nitrate reductase enzymes
Fig 2
MSMEG_4206 is not a pseudogene and has a distinct domain architecture.
(A) A schematic representation of the domain architecture of NarB (Accession: A0QW69) which is commonly found in bacterial NR enzymes. (B) Sequencing of the forward and reverse strand in the region of MSMEG_4206 predicted to contain a frameshift revealed that the frameshift is not present. (C) The distinct domain architecture of MSMEG_4206, as shown on InterPro (https://www.ebi.ac.uk/interpro/protein/). (D) The expression of each putative NR-encoding gene was measured in the presence of different nitrogen sources. The averages of three independent experiments with standard errors are shown. The Student’s t-test was performed for statistical analysis. *P< 0.005.