Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype
Fig 6
Implantation of three-dimensional human induced pluripotent stem cell-derived cardiac tissue (3D-hiPSC-CT) promoted extracellular matrix (ECM) remodeling and maturation of cardiomyocytes.
(A-1) Immunostaining of fibronectin (green) on 3D-hiPSC-CT in vitro. (A-2) Fibronectin expression (green) was poor in the cardiac tissue 12 weeks after transplantation. (B-1) Immunostaining of collagen IV (green) on the 3D-hiPSC-CT in vitro. (B-2) Collagen type IV (green) was clearly expressed in the transplanted tissue 12 weeks after implantation. (C-1) Immunostaining of perlcan (green) was not observed on the 3D-hiPSC-CT in vitro. (C-2) Perlcan (green) was clearly expressed in the transplanted tissue 12 weeks after implantation. (D-1) Immunostaining of desmin (green) was not observed on the 3D-hiPSC-CT in vitro. (D-2) Desmin (green) was expressed in the transplanted tissue. (E-1) Immunostaining of dystrophin (green) was not observed on the 3D-hiPSC-CT in vitro. (E-2) Dystrophin (green) was clearly expressed in the transplanted tissue. (F-1) The expression of connexin43 (green) was poor on the 3D-hiPSC-CT in vitro. (F-2) Connexin43 (green) was clearly expressed in the transplanted tissue 12 weeks after implantation.