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Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype

Fig 5

Ultrastructural analysis of 3D-hiPSC-CT using transmission electron microscopy (TEM).

(A) TEM images of 3D-hiPSC-CT in vitro. The hiPSC-CM (CM) are assembled to be in contact with each other via desmosomes (white arrow). A few myofibrils (mf) and mitochondria (m) are observed within the cardiomyocytes, scale bar = 1 μm. (B) TEM images of 3D-hiPSC-CT in vivo. The 3D-hiPS-CT showed clear thickened myofibrils (mf), Z-band (Z), and mitochondria (m). The adherens junction (black arrow) and desmosome (white arrow) were also disclosed between the iPSC-CM, scale bar = 1 μm.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0245571.g005