In situ kinetic measurements of α-synuclein aggregation reveal large population of short-lived oligomers
Fig 2
Room temperature EPR spectra of α-synuclein (R1-αS) at different time points of aggregation.
Full spectra: Inset, the box shows the region zoomed into. Zoomed-in spectra: Amplitude expanded four-fold with respect to inset. a) Start of aggregation (t = 0). b) 9 hours of aggregation. c) 24 hours of aggregation. d) 42 hours of aggregation. Black: Experimental spectra. Red: Simulated spectra. Arrow: feature of broad spectral component (see text).