Gut microbiome dysbiosis and correlation with blood biomarkers in active-tuberculosis in endemic setting
Fig 5
Correlations (Spearman) of anti-M. tb. antibodies in active-TB patients to gut microbiota.
Plasma antibodies against 11 M. tb. antigens in plasma samples from active TB patients are shown on X-axis. Microbiota, at the genus, are shown on Y-axis. The Spearman’s ranked correlation test with false discovery rate (FDR) adjustment was used to test the microbiome-antibody correlation. Antibodies against M. tb. Antigens that showed positive correlation with the enriched bacterial genera are shown is red and those that displayed negative correlations with the genera are shown in blue. Statistical analysis was not performed for Fig 5 since it shows a general comparison of microbiota profiling between TB patients and healthy individuals. Microbiota most common in TB patients contain antibodies agaist antigens Rv0934, Rv1926c, Rv1860, Rv3841, and Rv1886c in addition to the whole membrane extract from H37Rv bacteria. The clusters that were boxed and specifically highlighted were identified visually based on the differences between healthy individuals and TB patients. Green box shows the microbiota profiles common in TB patients when the antibodies agaist 6 M.tb. antigens mentioned above are detected. Red box represents the microbiota profiling of healthy individuals who did not have antibodies against these antigens.