Gut microbiome dysbiosis and correlation with blood biomarkers in active-tuberculosis in endemic setting
Fig 2
Gut microbiota diversity in tuberculosis patients.
(A) Principal coordinates analysis of unweighted UniFrac distance based on 16S rDNA profiling of the gut microbiome from healthy (n = 40) and tuberculosis cohorts (n = 42), p value statistically significant based on Bonferroni-corrected parametric t-test, ****p< 0.001. Gut microbiome in active-TB patients (red) and healthy individuals (blue) display separation in the two groups. Each data point represents the entire microbial signature in individual samples. (B) Shannon diversity index of fecal 16S rDNA sequencing data from healthy (n = 40) and tuberculosis cohorts (n = 42). p value statistically significant based on Man-Whitney t-test, **p< 0.01. (C) Microbiome profiles (84 genera and families where unknown genus contributed to significant difference in comparison with healthy controls) of microbiota shown in a heat map. Unique clusters of genera in active-TB patients are outlined by a yellow box and those in age-matched healthy individuals by the green boxes. Each small square in the heat map represents a genus. Clusters of bacterial genera in TB patients are well separated from those in healthy individuals.