Platelets express adaptor proteins of the extrinsic apoptosis pathway and can activate caspase-8
Fig 5
ITP plasma treatment of platelets derived from healthy donors show no significant caspase- 3/7, and -8 activation.
Flow cytometry analyses of healthy platelets treated with ITP (n = 3), healthy (n = 2) and autologous plasma (n = 2) and A23187 (n = 2). Healthy platelets were incubated for 2h with ITP plasma at 37°C. FLICA% platelets are: (A) FLICA reagent, FAM-DEVD-FMK, irreversibly bound to active caspase- 3/7, and (B) FLICA reagent, FAM-LETD-FMK, irreversibly bound to active caspase- 8 (B), both measured as green fluorescent signal. Autologous plasma was used as a negative control. Statistical analyses were performed using one-way ANOVA followed by multiple comparisons tests to compare the mean ranks between groups. Data are presented as Standard Error of the Mean (SEM).