Platelets express adaptor proteins of the extrinsic apoptosis pathway and can activate caspase-8
Fig 2
FADD and DJ-1 co-localize and possibly interact in human platelets.
(A) Representative confocal images show the co-localization of FADD (green) and DJ-1 (red) resulting in a yellow stain (white arrows). Shown is one confocal plane, objective 63x glycerol with a numerical aperture of 1.3. Images were processed by using confocal laser scanning microscope SP8 (Leica). We also calculated a colocalization correlation coefficient Pearson’s R value of 0.88 with Image J software. Scale bar 10μm. (B) co-IP of FADD and DJ-1 in human PCs. As control the whole platelet lysate was immunoblotted (Input) and IgG controls were used to determine unspecific binding of the protein to the antibody (n = 3). Immunoblot 1 (WB 1 top): FADD probed; Immunoblot 2 (WB 2 bottom): DJ-1 probed.