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Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation

Fig 2

The cytotoxicity of GMI on C2C12 myoblasts.

(A) C2C12 cells were treated with different dosage of GMI (0, 0.01, 0.05, 0.1, 0.5, 1, 5, 10, 20 and 30 μg/ml) for 24 hours and the morphological changes were visualized. (B) The cell viability of GMI pre-treated C2C12 was determined by using CCK-8 assay. Statistical analyses were performed by One-way ANOVA. ***, p < 0.001 and **** p < 0.0001.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0244791.g002