Modeling dysbiosis of human NASH in mice: Loss of gut microbiome diversity and overgrowth of Erysipelotrichales
Fig 2
Loss of abundance and altered composition of microbiome in NASH mice.
Feces were collected from chow-fed controls or NASH mice at weeks 0, 12, and 24. Composition of the microbiome was assessed 16S rRNA amplicon sequencing. A) Microbiota density in feces. NASH mice had significantly lower bacterial density than controls. B) Faith’s phylogenetic diversity, calculated to assess species diversity. NASH mice displayed reduced alpha diversity. C) Principal Coordinate Analysis plotting weighted UniFrac distances. NASH mice at 12 and 24 weeks cluster separately from week 0 baseline or chow-fed controls. (A-C) fecal samples from 3–7 mice per condition, per timepoint. P values are encoded as * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.