Memory impairment in chronic experimental Chagas disease: Benznidazole therapy reversed cognitive deficit in association with reduction of parasite load and oxidative stress in the nervous tissue
Fig 5
CNS tissue architecture is preserved in chronically Trypanosoma cruzi-infected C57BL/6 mice.
Mice were infected with 100 blood trypomastigote forms of the Colombian T. cruzi strain and benznidazole (Bz, 0.1 mL gavage; 25 mg/Kg/day) or pyrogen-free water (Veh, 0.1 mL gavage) were administered for 30 consecutive days, from 120 to 150 dpi, when mice were euthanized, tissue collected and fixed. Tissue sections were stained with hematoxylin and eosin. (A) Representative sections of cerebral cortex, hippocampus, and cerebellum areas of noninfected, and Veh-treated and Bz-treated T. cruzi-infected mice. Bar = 100 μm. (B) Representative sections of choroid plexus areas of noninfected, and Veh-treated and Bz-treated T. cruzi-infected mice. Bar = 100 μm. Arrows show infiltration of rare mononuclear inflammatory cells in perivascular areas.