Three dimensional cultivation increases chemo- and radioresistance of colorectal cancer cell lines
Fig 5
Upon 5-FU treatment the DNA repair pathway is activated stronger in 2D cultures.
2D and 3D cultures of HCT116 and SW480 cell lines were treated with 5-FU at the indicated concentrations for 24h. Afterwards, cells were harvested and analysed by immunoblotting. The size of the proteins is shown on the left. Figure shows representative blots from three independent experiments.