Three dimensional cultivation increases chemo- and radioresistance of colorectal cancer cell lines
Fig 2
Histological analyses confirm loss of integrity and proliferation.
CRC cell lines were grown in matrigel as 3D spheroids and irradiated with an Elekta Versa HD accelerator. Six days after irradiation, spheroids were fixed with 4% PFA, embedded in paraffin, and stained with (A) H&E or (B) Ki-67 (scale bar 100μm).