Informed sequential pooling approach to detect SARS-CoV-2 infection
Fig 2
Graphic representation of the Informed sequential pooling approach.
The cohort dimension is N = 30, the pool size is 5, and the vf is 0.10. Panels A and B show two possible scenarios based on available information allowing for the classification of subjects as either “suspect positive” or “suspect negative.” In scenario A, information allows for a concentration of all the positive subjects in the same horizontal pool. Thus after 6H pools, only 5 subjects are left, and vertical pooling is not necessary (globally 11 tests are required). In scenario B, information allows for a concentration of some of the positive samples but not all of them. Among 6 horizontal pools, 4 turn out to be negative. The 10 remaining subjects are then rearranged in 2 vertical pools. Since one pool gives a positive result, 5 validation tests are required (globally 13 tests).