A strategic expression method of miR-29b and its anti-fibrotic effect based on RNA-sequencing analysis
Fig 7
Differentially Expressed Gene (DEG) profiling in MEF cells.
MEF cells transfected with empty vector, col1a1-miR-29b vector, or CMV-miR-29b vector, then treated with 20 ng/ml TGF-β for 24h. The total RNA was extracted for RNA-sequencing by LC Science. (A) The log2 foldchange of miR-29b expression cells in transfected cells. The log2 fold change and p-value were calculated using R-package DESeq2 as described in the Method section. (B) Volcano plot of significantly regulated genes after TGF-β treatment. (C) Heatmap of DEG in different groups.