A strategic expression method of miR-29b and its anti-fibrotic effect based on RNA-sequencing analysis
Fig 3
Infection by lentiviral constructs in Mouse Embryonic Fibroblast (MEF) cells.
(A) The CMV-RFP/col1a1-EGFP vector was packed into lentivirus and mixed with polybrene. (B) MEF cells were cultured on a 6-well plate for 24h and infected with the lentiviruses for 48h. EGFP and RFP were visualized under a fluorescence microscope with a 20x lens.